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Factors affecting enzyme activity
Substrate concentration- must be in excess to maintain 0 order
product concentration- shifts equilibrium, causes same effect as substrate depletion if in excess
coenzyme concentration- Acts like substrate (NAD), if not enough of it behaves like substrate depletion ->1st order
concentration of Activators/cofactors- (Calcium, magnesium, vitamins) some systems require it to do anything, but if depleted causes 1st order
pH- Most enzymes prefer a fairly neutral pH (7-7.4), but sometimes they have much different pH's that they work at
Temperature- works best at 37 degrees C as temp increases, activity increases, but when the temp increases too uch the bonds deteriorate
Stability- stability of enzymes are variable, some need a specific temp (acid phosphatase) but others can sit for days (amylase)
Anticoagulant- should be avoided, they bind to Calcium and usualy Mg, use heperin in coagulations beause it doesn't use Ca
Hemolysis- enzymes reacting in plasma instead of cells, usually bec of bad collection
Inhibitors
product concentration- shifts equilibrium, causes same effect as substrate depletion if in excess
coenzyme concentration- Acts like substrate (NAD), if not enough of it behaves like substrate depletion ->1st order
concentration of Activators/cofactors- (Calcium, magnesium, vitamins) some systems require it to do anything, but if depleted causes 1st order
pH- Most enzymes prefer a fairly neutral pH (7-7.4), but sometimes they have much different pH's that they work at
Temperature- works best at 37 degrees C as temp increases, activity increases, but when the temp increases too uch the bonds deteriorate
Stability- stability of enzymes are variable, some need a specific temp (acid phosphatase) but others can sit for days (amylase)
Anticoagulant- should be avoided, they bind to Calcium and usualy Mg, use heperin in coagulations beause it doesn't use Ca
Hemolysis- enzymes reacting in plasma instead of cells, usually bec of bad collection
Inhibitors
