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NAD / NADH and light absorption
both absorb light in 265 nm, but at 340 only NADH absorbs lights.
If you start with NAD and change to NADH the absorbance will increase with time. The more enzyme you have that converts it, the faster it will go.
IF you start with NADH and go to NAD the absorbance decreases with time and bottoms off when there's no more to convert.
If you start with NAD and change to NADH the absorbance will increase with time. The more enzyme you have that converts it, the faster it will go.
IF you start with NADH and go to NAD the absorbance decreases with time and bottoms off when there's no more to convert.
