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turbidimetry
principle: reduction of light transmitted (light blocked)
protein must be exposed to weak acid to create particulate proteins suspended
sources error: # and cross sectional area of particles, timing (longer you let sit, the more transmittance), temp- high temp makes particulates faster and more likely to deflect light, problems with alb/ glob ratio
sensitivity low concentrations (mg/dL) good for urine and spinal fluid
protein must be exposed to weak acid to create particulate proteins suspended
sources error: # and cross sectional area of particles, timing (longer you let sit, the more transmittance), temp- high temp makes particulates faster and more likely to deflect light, problems with alb/ glob ratio
sensitivity low concentrations (mg/dL) good for urine and spinal fluid
Karteninfo:
Autor: skunz11
Oberthema: Bio Chemisty
Thema: Clinical Bio Chem
Veröffentlicht: 02.03.2010